My research focuses on development of highly efficient and specific genome editing tools
including the synthetic site specific nucleases TALENs and CRISPR/Cas9 system. TALENs,
also known as "molecular scissors", are composed of an adaptable DNA binding module fused to a
catalytic nuclease domain. The DNA binding module can be engineered to recognize any given DNA
sequence, whereas the catalytic module introduces double stranded breaks on the DNA. The efficiency
of this platform largely depends on the adaptability or customizability of the DNA binding module.
The Cas9 system is directed by single guide RNA molecule to its genomic target and can be easily
reprogrammed and customized to cleave any user selected DNA target. The easy programmability and
customization of the RNA molecule, which directs the Cas9 nuclease, holds significant potential
for improving the quality and yield of current crop varieties. Using both technologies, novel
genes can be identified, crop trait development can be accelerated, and the range of possible
traits can be expanded.
Research Interests Keywords
Targeted genome editing Synthetic nucleases
CRISPR/Cas9 System TALENs
Li L, Atef A, Piatek A, Ali Z, Piatek M, Aouida M, Sharakuu A, Mahjoub A, Wang G, Khan S, Fedoroff NV, Zhu JK, Mahfouz MM. “Characterization and DNA-binding specificities of Ralstonia TAL-like effectors.” Mol Plant. 2013 Jul;6(4):1318-30. doi: 10.1093/mp/sst006. Epub 2013 Jan 8.
Li L, Piatek MJ, Atef A, Piatek A, Wibowo A, Fang X, Sabir JS, Zhu JK, Mahfouz MM. “Rapid and highly efficient construction of TALE-based transcriptional regulators and nucleases for genome modification.” Plant Mol Biol. 2012 Mar;78(4-5):407-16. doi: 10.1007/s11103-012-9875-4. Epub 2012 Jan 22
February 2011 – present:PhD Student, King Abdullah University of Science and Technology, Saudi Arabia.
2009: MSc in Microbial and Plant Biotechnology, University of Silesia, Poland.
2008: BSc (Hons) in Molecular and Cellular Biology, 1st Class, University of Huddersfield, United Kingdom.
2006: BSc in Microbial and Plant Biotechnology, University of Silesia, Poland.
2011: Cutting-edge Bioinformatics workshop, BGI, China
2009: Research Intern at Cranfield University, United Kingdom
2008-2009:Member of Association of Biotechnology Students at University of Silesia, Pol Silesia, Poland
2003: Volunteer at the Maria Sklodowska-Curie Comprehensive Cancer Centre, Poland
2001:KAUST Provost Award
2009:Socrates Erasmus Scholarship
2007:Scholarship for academic performance at University of Silesia, Poland
2004:Scholarship for academic performance at University of Silesia, Poland