Accelerated genome evolution holds great promise for a variety of biotechnological applications in agriculture, industry and medicine. Our major interest is to use the Transcription Activator-Like Effectors Nucleases (TALENs) to generate genomic variants in yeast for metabolic engineering and study basic biological questions like DNA repair and cell growth control. This surrogate system will serve different purposes including:
To rapidly validate the TALEN function in yeast,
Study the mechanism of DNA binding and recognition by TALENs,
Provide proof of principal to generate genomic variants.
Knock down toxic genes in domesticated plants like Salicornia saponins.
Biofuel overproduction from microorganisms such as yeast and microalgae.
Research Interests Keywords
Genome engineering Yeast genetics and molecular biology
Metabolic engineering Microbiology
Mustapha Aouida, Kangchang Kim, AbdulRajjak S Shaikh, Jose M. Pardo, Jorg Eppinger, Ray A. Bressan, Meena L. Narasimhan. A split luciferase method to identify Adiponectin receptors agonists using Saccharomyces cerevisiae as a model organism. PLoS One. 2013 Jun 7;8(6):e65454. doi: 10.1371/journal.pone.0065454. Print 2013
Mustapha Aouida, Marta Rubio Texeira, Johan M. Thevelein, Richard Poulin and Dindial Ramotar. AGP2 Encodes a Multifunctional Transceptor that Positively Regulates Polyamine Transport.PLoS One. 2013 Jun 3;8(6):e65717. doi: 10.1371/journal.pone.0065717. Print 2013
Mustapha Aouida, Anick Leduc, Richard Poulin and Dindial Ramotar. AGP2 encodes the major permease for high affinity polyamine import in Saccharomyces cerevisiae. J Biol Chem. (2005): 280(25): 24267-24276
Mustapha Aouida, Nicolas Page, Anick Leduc, Matthias Peter and Dindial Ramotar. A genome-wide screen in Saccharomyces cerevisiae reveals altered transport as a major mechanism of resistance to the anticancer drug bleomycin. Cancer Res. 2004 Feb 1; 64(3):1102-9.
2003: PhD in Biological Sciences (Option: Microbiology) Gustave Roussy Institute, Paris (France), Science University of Tunis, Tunisia
1999: Master's Degree in Microbiology Science University of Tunis, Tunisia
1997: Bachelor in Natural Sciences, University of Tunis, Tunisia
November 2010 - present: Postdoctoral fellow, Genome |Engineering Group, Biosciences,King Abdullah University of Science Technology, KAUST, KSA
2008 - 2010: Research Associate, Laboratory Department of Oncology and Immunology, Maisonneuve-Rosemont Research Center Hospital, University of Montreal
2003 - 2008: Postdoctoral Fellow, Laboratory Department of Oncology and Immunology, Maisonneuve-Rosemont Research Center Hospital, University of Montreal
Scientific and Professional Membership
2013-2014: International Metabolic Engineering Society (IMES)
2011 - 2013 : Genetics society of America
2009 -2012: Canadian Society for Biochemistry and Molecular & Cellular Biology
2007 -2011: Yeast Genome database member
1997 -1999: French Association of Biological Sciences
2000 –2002: Tunisian Association of Biochemistry and Molecular Biology
2009-2012: Canadian society of molecular biosciences
2005 - 2008: Post PhD fellowship from National Cancer Institute of Canada
2003 - 2005: Scholarship from Maisonneuve-Rosemont Foundation, Montreal (University of Montreal)
1999 - 2003: Scholarship from Tunisian Government and second scholarship from CNRS-France